文章成果

Method of the Year 2015

Biological Cryo-EM
  • Nature Methods 13:1 - 32 (2016)

Eisenstein M, Doerr A, Nogales E, Glaeser RM    

Abstract: Nature Methods has named single-particle cryo-electron microscppy the Method of the Year for 2015 based on “impressive leaps in achievable resolution” due to “recent technical advances, especially the development of direct-detection...



Structure and membrane remodeling activity of ESCRT-III helical polymers

Biological Cryo-EM
  • Science 350: 1548 - 1551 (2015)

McCullough J, Clippinger AK, Talledge N, Skowyra ML, Saunders MG, Naismith TV, Colf LA, Afonine P, Arthur C, Sundquist WI, Hanson PI, Frost A

A 4 Å cryo-EM reconstruction of a double-stranded helical copolymer of two different human ESCRT-III subunits. The cryo-EM data set was merged from multiple data sets acquired on several different microscopes, including a JEOL 3200FSC with a DE-12 camera.



In Situ Study of Lithiation and Delithiation of MoS2 Nanosheets Using Electrochemical Liquid Cell Tr

In situ TEM
  • Nano Letters 15: 5214 - 5220 (2015)

Zeng Z, Zhang X, Bustillo K, Niu K, Gammer C, Xu J, Zheng H

Abstract:Observation of lithiation/delithiation of MoS2 nanosheets in a LiPF6/EC/DEC commercial electrolyte for the application of lithium-ion batteries using electrochemical liquid cell transmission electron microscopy (TEM) with a DE-12 Camera.



The influence of frame alignment with dose compensation on the quality of single particle reconstruc

Biological Cryo-EM
  • Journal of Structural Biology In press: (2015)

Spear JM, Noble AJ, Xie Q, Sousa DR, Chapman MS, Stagg SM

Abstract:A 2.8 Å resolution cryo-EM structure of a recombinant gene therapy virus, AAV-DJ with Arixtra, from an FEI Titan Krios with a DE-20 Camera System. Several metrics are also presented for optimizing the compensation of beam damage and characterizing the factors that are limiting...



Three-Dimensional Structural Characterization of HIV-1 Tethered to Human Cells

Biological Cryo-EM
  • Journal of Virology 90: 1507 - 1521 (2015)

Strauss JD, Hammonds JE, Yi H, Ding L, Spearman P, Wright ER

Abstract:Cryo-electron tomography (cryo-ET) of HIV-1 virus-like particles (VLPs) and virions tethered to human cells. Reconstructions showed the spatial arrangement and orientation of tetherin molecules at sites of HIV-1 restriction.



Decontamination of digital image sensors and assessment of electron microscope performance in a BSL-

  • AIMS Biophysics 2: 153-62 (2015)

Sherman M, Trujillo J, Bammes B, Jin L, Stumpf M, Weaver S

Abstract:Tests of digital camera sensors (both CCD and CMOS direct detector) in a BSL-3 environment, and microscope performance after chlorine dioxide (ClO2) decontamination cycles.



A Newly Isolated Reovirus Has the Simplest Genomic and Structural Organization of Any Reovirus

Biological Cryo-EM
  • Journal of Virology 89: 676 - 687 (2015)

Auguste AJ, Kaelber JT, Fokam EB, Guzman H, Carrington CV, Erasmus JH, Kamgang B, Popov VL, Jakana J, Liu X, Wood TG, Widen SG, Vasilakis N, Tesh RB, Chiu W, Weaver SC

Abstract:The detection, genetic, phenotypic, and structural characteristics of a novel Dinovernavirus species isolated from mosquitoes collected in Cameroon, tentatively designated Fako virus (FAKV).



Catadioptric aberration correction in cathode lens microscopy

  • Ultramicroscopy 151: 191-8 (2015)

Tromp RM

Abstract:A review of the use of electrostatic electron mirrors to correct the aberrations of the cathode lens objective lens in low energy electron microscope (LEEM) and photo electron emission microscope (PEEM) instruments.



Automated batch fiducial-less tilt-series alignment in Appion using Protomo

Biological Cryo-EM
  • Journal of Structural Biology In press: (2015)

Noble AJ, Stagg SM

Abstract:A set of automated tilt-series alignment, contrast transfer function (CTF) correction, and reconstruction workflows for use in conjunction with the Appion/Leginon package, which are primarily targeted at automating structure determination with electron cryo-tomography.



Visualization of Electrode–Electrolyte Interfaces in LiPF6/EC/DEC Electrolyte for Lithium Ion Batter

  • Nano Letters 14: 1745-1750 (2014)

Zeng Z, Liang WI, Liao HG, Xin HL, Chu YH, Zheng H

Abstract:The first published in situ TEM study with a DDD, showing direct visualization of electrochemical lithiation and delithiation of Au anodes.