文章成果

Structure of the AcrAB–TolC multidrug efflux pump

  • Nature 509: 512-515 (2014)

Du D, Wang Z, James NR, Voss JE, Klimont E, Ohene-Agyei T, Venter H, Chiu W, Luisi BF

Abstract:The pseudo-atomic structure of a complete multidrug efflux pump in complex with a modulatory protein partner from E. coli.



Crystal structure of a nematode-infecting virus

Biological Cryo-EM
  • Proceedings of the National Academy of Sciences 111: 12781 - 12786 (2014)

Guo YR, Hryc CF, Jakana J, Jiang H, Wang D, Chiu W, Zhong W, Tao YJ

Abstract:The crystal structure and cryo-EM 3D reconstruction of an Orsay virus-like particle assembled from recombinant capsid protein (CP).



An atomic model of brome mosaic virus using direct electron detection and real-space optimization

Biological Cryo-EM
  • Nature Communications 5: 4808 (2014)

Wang Z, Hryc CF, Bammes B, Afonine PV, Jakana J, Chen DH, Liu X, Baker ML, Kao C, Ludtke SJ, Schmid MF, Adams PD, Chiu W

Abstract:A rigorously-validated atomic model of brome mosaic virus (BMV) from single-particle cryo-EM using a DDD an damage compensation.



Three-dimensional reconstructions of the bacteriophage CUS-3 virion reveal a conserved coat protein

Biological Cryo-EM
  • Virology 464-465: 55-66 (2014)

Parent KN, Tang J, Cardone G, Gilcrease EB, Janssen ME, Olson NH, Casjens SR, Baker TS

Abstract:Icosahedrally averaged and asymmetric 3D cryo-EM reconstructions of the CUS-3 virion. The structure is compared to other P22-like phages.



In Silico, In Vitro, and In Vivo Studies Indicate the Potential Use of Bolaamphiphiles for Therapeut

  • Molecular Therapy Nucleic Acids 2: e80 (2013)

Kim T, Afonin KA, Viard M, Koyfman AY, Sparks S, Heldman E, Grinberg S, Linder C, Blumenthal RP, Shapiro BA

Abstract:Two different bolaamphiphiles are compared for their abilities to complex with siRNAs and deliver them into cells inducing gene silencing.



Noise models and cryo-EM drift correction with a direct-electron camera

  • Ultramicroscopy 131: 61-69 (2013)

Shigematsu H, Sigworth FJ

Abstract:A filter that includes a model of noise from “hot” pixels is derived. This optimizes DDD motion correction by cross-correlation.



Allosteric Regulation of DNA Cleavage and Sequence-Specificity through Run-On Oligomerization

Biological Cryo-EM
  • Structure 21: 1848-1858 (2013)

Lyumkis D, Talley H, Stewart A, Shah S, Park CK, Tama F, Potter CS, Carragher B, Horton NC

Abstract:3D reconstructions of distinct populations of SgrAI oligomers, showing repeating helical structures in the presence of activating DNA.



Quantitative characterization of electron detectors for transmission electron microscopy

  • Journal of Structural Biology 184: 385-393 (2013)

Ruskin RS, Yu Z, Grigorieff N

Abstract:A simple software tool for measuring MTF and DQE of electron detectors using a beam stop. DQE curves of various cameras are shown.



Improving Signal to Noise in Labeled Biological Specimens Using Energy-Filtered TEM of Sections with

  • Microscopy & Microanalysis 20: 706-714 (2013)

Ramachandra R, Bouwer JC, Mackey MR, Bushong E, Peltier ST, Xuong NH, Ellisman MH

Abstract:EFTEM imaging using a DDD with motion correction has a 3× improvement in signal compared to a similarly-formatted CCD.



Beam-induced motion of vitrified specimen on holey carbon film

  • Journal of Structural Biology 177: 630-637 (2012)

Brilot AF, Chen JZ, Cheng A, Pan J, Harrison SC, Potter CS, Carragher B, Henderson R, Grigorieff N

Abstract:DDD movies show that the specimen moves significantly during an exposure. Alignment of movie frames can restore high-resolution detail.